RealHelix™ qPCR Kit [Probe] 는 target DNA 검출을 위해 probe를 사용하는 실시간 정량 PCR (real-time PCR)에 적합한 제품입니다. 제공되는 2x 농도의 premix에는 hot-start PCR enzyme, dNTPs, buffer, Mg²⁺ 및 안정제(stabilizers)가 혼합되어 있습니다. Hot-start PCR enzyme을 적용하여 target DNA에 대한 증폭 특이도가 매우 높고(high specificity), 비특이적(non-specific) 증폭 및 primer dimer 생성을 최소화할 수 있습니다.

Figure 1. The accuracy of real-time PCR using RealHelix™ qPCR Kit [Probe].

Human genomic DNAs (10, 1, 0.1, and 0.01 ng) isolated from whole blood were analyzed using the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene-specific primer set and 5’-FAM-labelled probe. The accuracy of real-time PCR is demonstrated by E=99.9 %, R² value=0.997. Performed on a CFX96 real-time PCR detection system (Bio-Rad).

Figure 2. Comparison of real-time PCR using RealHelix™ qPCR Kit [Probe] and its previous version (qPCR Kit [Probe type]).

Human genomic DNAs (100, 10, 1, and 0.1 ng) isolated from whole blood were analyzed using the beta-globin gene-specific primer set and 5’-Quasar705-labelled probe. Performed on a CFX96 real-time PCR detection system (Bio-Rad).

Storage

• Store below -20 ℃
• ROX dye should be stored in a dark place.

Shelf life

• 12 months